Can my 2-slot motherboard handle 2x16GB of RAM?

Yes, in most cases. For any modern DDR4 or DDR5 motherboard with two slots, 32GB (2x16GB) is a standard supported configuration, and many even go up to 64GB. But check your motherboard’s official specs — if it says “Max memory: 32GB,” you’re fine; if it says “16GB” (very old boards), you’re stuck with 2x8GB. Also make sure your CPU supports the density, and buy a matched kit to avoid dual-channel headaches.